FRET Single-Band Sets
FRET Single-Band Sets
產品名稱 : FRET Single-Band Sets
產品說明 :
Fluorescence (or Förster) Resonance Energy Transfer (FRET) is a powerful technique for characterizing distance-dependent interactions on a molecular scale. FRET starts with the excitation of a “donor” fluorophore molecule by incident light within its absorption spectrum. If another fluorophore molecule (the “acceptor”) is in close proximity to the donor and has an absorption spectrum that overlaps the donor emission spectrum, nonradiative energy transfer may occur between donor and acceptor. For example, CFP and YFP support a strong FRET interaction. FRET can measure distances on the order of the “Förster distance” – typically 20 to 90 Å. This length scale is far below the Rayleigh-criterion resolution limit of an optical microscope (about 2500 Å for visible light and high numerical aperture), thus llustrating the power of FRET for measuring extremely small distance interactions.
| 產品型號 | 產品說明 | Primary Fluorophores | Excitation (CWL/BW) | Emission (CWL/BW) | Dichroic (Edge) | 價格(NTD) | 產品數量 | |
|---|---|---|---|---|---|---|---|---|
| 0 | FRET-BFP-GFP-B-000 | BrightLine® FRET Filter Set | Blue and Green Fluorescent Protein | 387 / 11 nm | Em1: 447 / 60 nm; Em2: 520 / 35 nm | 409 nm | - | |
| 1 | FRET-CFP-YFP-C-000 | BrightLine® FRET Filter Set | Cyan and Yellow Fluorescent Protein | 438 / 24 nm | Em1: 483 / 32 nm; Em2: 542 / 27 nm | 458 nm | - | |
| 2 | FRET-GFP-RFP-D-000 | BrightLine® FRET Filter Set | Green and Red Fluorescent Protein | 472 / 30 nm | Em1: 520 / 35 nm; Em2: 641 / 75 nm | 495 nm | - | |
| 3 | FRET-CY3-CY5-A-000 | BrightLine® FRET Filter Set | Cy3 and Cy5 | 531 / 40 nm | Em1: 593 / 40 nm; Em2: 676 / 29 nm | 562 nm | - |